-
Purpose(Empty Backbone) For expression of sgRNA from the U6 promoter with EGFP expression simultaneously
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107721 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepGL3-U6-sgRNA-PGK-puromycin
-
Vector typeCRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGL3-U6-sgRNA-EGFP was a gift from Xingxu Huang (Addgene plasmid # 107721 ; http://n2t.net/addgene:107721 ; RRID:Addgene_107721) -
For your References section:
Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing. Liu Z, Lu Z, Yang G, Huang S, Li G, Feng S, Liu Y, Li J, Yu W, Zhang Y, Chen J, Sun Q, Huang X. Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7. 10.1038/s41467-018-04768-7 PubMed 29904106
