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Addgene

pN3-Nipbl
(Plasmid #107716)

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Item Catalog # Description Quantity Price (USD)
Plasmid 107716 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pN3-3xFlag
  • Backbone size w/o insert (bp) 4063
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NIPBL
  • Alt name
    NIPBL cohesin loading factor
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    8385
  • Entrez Gene
    Nipbl (a.k.a. Idn3)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-for (CGCAAATGGGCGGTAGGCGTG)
  • 3′ sequencing primer SV40-pA-rev (CCTCACAAATGTGGTATGG)
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid encodes the 2798 amino acid full-length murine NIPBL

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN3-Nipbl was a gift from Guntram Suske (Addgene plasmid # 107716 ; http://n2t.net/addgene:107716 ; RRID:Addgene_107716)
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