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pAAV-EF1alpha-soCoChR-tdTomato
(Plasmid #107714)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107714 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    soCoChR-tdTomato
  • Alt name
    CoChR-ggsggtggsggt-KA2-(1-150)
  • Alt name
    Chloromonas oogama channelrhodopsin, fused to KA2 targeting sequence
  • Species
    Chloromonas oogama
  • Promoter EF1alpha
  • Tags / Fusion Proteins
    • KA2 (C terminal on insert)
    • tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank IDs: soCoChR = MF795583, KA2(1-150) = MF795584

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1alpha-soCoChR-tdTomato was a gift from Edward Boyden (Addgene plasmid # 107714 ; http://n2t.net/addgene:107714 ; RRID:Addgene_107714)

  • For your References section:

    Temporally precise single-cell-resolution optogenetics. Shemesh OA, Tanese D, Zampini V, Linghu C, Piatkevich K, Ronzitti E, Papagiakoumou E, Boyden ES, Emiliani V. Nat Neurosci. 2017 Dec;20(12):1796-1806. doi: 10.1038/s41593-017-0018-8. Epub 2017 Nov 13. 10.1038/s41593-017-0018-8 PubMed 29184208
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