pAAV CAGG eGFP
(Plasmid
#107707)
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Purposeexpress eGFP in variety of cell types
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107707 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-MCS
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Backbone manufacturerAgilent
- Backbone size w/o insert (bp) 3500
- Total vector size (bp) 5947
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsgrow according to manufacturers instructions
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP
- Promoter CAGG
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer ggagggccttcgtgcgt
- 3′ sequencing primer CAAGGCTGGTGGGCACTGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV CAGG eGFP was a gift from Troy Margrie (Addgene plasmid # 107707 ; http://n2t.net/addgene:107707 ; RRID:Addgene_107707) -
For your References section:
A Circuit for Integration of Head- and Visual-Motion Signals in Layer 6 of Mouse Primary Visual Cortex. Velez-Fort M, Bracey EF, Keshavarzi S, Rousseau CV, Cossell L, Lenzi SC, Strom M, Margrie TW. Neuron. 2018 Apr 4;98(1):179-191.e6. doi: 10.1016/j.neuron.2018.02.023. Epub 2018 Mar 15. 10.1016/j.neuron.2018.02.023 PubMed 29551490