pAAV CAGG eGFP
(Plasmid #107707)

• Purpose: express eGFP in variety of cell types
• Depositing Lab: Troy Margrie
• Publication: Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi: 10.1016/j.neuron.2018.02.023. Epub 2018 Mar 15.

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Backbone

• Vector backbone: pAAV-MCS
• Backbone manufacturer: Agilent
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 5947
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: grow according to manufacturers instructions
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP
• Promoter: CAGG

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: ggagggccttcgtgcgt
• 3′ sequencing primer: CAAGGCTGGTGGGCACTGG

Resource Information

• Supplemental Documents:
  • pAAV-CAGG-EGFP.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry