pmCherry-Zdk1-EB1C
(Plasmid #107695)

• Purpose: C-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue light
• Depositing Lab: Torsten Wittmann
• Publication: van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038/s41556-017-0028-5. doi: 10.1038/s41556-017-0028-5.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3988
• Total vector size (bp): 5155
• Modifications to backbone: removed EGFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MAPRE1
• Alt name: EB1
• Species: H. sapiens (human)
• Insert Size (bp): 1167
• Mutation: EB1 aa 186-268
• GenBank ID: NM_012325.2
• Entrez Gene: MAPRE1 (a.k.a. EB1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCherry (N terminal on insert)
  • Zdk1 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: SV40 reverse

Resource Information

• Supplemental Documents:
  • mCherry-Z-linker EB1-C.gbk
• A portion of this plasmid was derived from a plasmid made by: EB1 from L. Cassimeris (Addgene #17234) Zdk1 from Klaus Hahn (Addgene #81057)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry