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Addgene

pJJB296
(Plasmid #107691)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107691 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMOD_A
  • Total vector size (bp) 6626
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas13a
  • Alt name
    C2c2
  • Species
    dicot
  • Promoter 35S

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer gagagaacacgggggactccCGACATGGGATCCATGAAAGTGACCAAGG
  • 3′ sequencing primer CATGTTCGAGTACAAGGCCCTGGAATGAcgacgatatgaagatgaaga
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Feng Zhang at MIT
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a MOD_A plasmid compatible with the Cermek et al. toolkit.
https://www.addgene.org/browse/article/28189956/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJB296 was a gift from Daniel Voytas (Addgene plasmid # 107691 ; http://n2t.net/addgene:107691 ; RRID:Addgene_107691)

  • For your References section:

    RNA targeting with CRISPR-Cas13. Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F. Nature. 2017 Oct 4. doi: 10.1038/nature24049. 10.1038/nature24049 PubMed 28976959
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