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Addgene

pCMV-dCpf1-eBE
(Plasmid #107688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dLbCpf1-eBE
  • Alt name
    pCMV-SV40NLS-APOBEC1-XTEN-dLbCpf1(D832A/E1006A/D1125A)-SV40NLS-SGGS-UGI-SV40NLS-T2A-UGI-SV40NLS-P2A-UGI-SV40NLS-T2A-UGI-SV40NLS
  • Species
    R. norvegicus (rat); Lachnospiraceae bacterium, Bacteriophage PBS2
  • Insert Size (bp)
    5757
  • Mutation
    dLbCpf1(D832A/E925A/D1148A)
  • Promoter CMV

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    derived from plasmid pCMV-BE3 (David Liu, Addgene plasmid # 73021)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

derived from plasmid pCMV-BE3 (David Liu, Addgene plasmid # 73021)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-dCpf1-eBE was a gift from Jia Chen (Addgene plasmid # 107688 ; http://n2t.net/addgene:107688 ; RRID:Addgene_107688)

  • For your References section:

    Base editing with a Cpf1-cytidine deaminase fusion. Li X, Wang Y, Liu Y, Yang B, Wang X, Wei J, Lu Z, Zhang Y, Wu J, Huang X, Yang L, Chen J. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. 10.1038/nbt.4102 PubMed 29553573
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