pCMV-dLbCpf1-BE0ΔiNLS
(Plasmid #107684)

• Purpose: Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells
• Depositing Lab: Jia Chen
• Publication: Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.

Backbone

• Vector backbone: CMV
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dLbCpf1-BE0ΔiNLS
• Alt name: pCMV-APOBEC1-XTEN-dLbCpf1(D832A/E1006A/D1125A)-SGGS-UGI-SV40NLS
• Species: R. norvegicus (rat); Lachnospiraceae bacterium, Bacteriophage PBS2
• Insert Size (bp): 4719
• Mutation: D832A/E925A/D1148A
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGH poly reverse

Resource Information

• Supplemental Documents:
  • pCMV-dLbCpf1-BE0ΔiNLS.gb
• A portion of this plasmid was derived from a plasmid made by: derived from plasmid pCMV-BE3 (David Liu, Addgene plasmid # 73021)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

derived from plasmid pCMV-BE3 (David Liu, Addgene plasmid # 73021)