Ddo_slit-1
(Plasmid
#107636)
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PurposeIn situ hybridization and RNAi studies in planarians
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107636 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPR-T4P
- Backbone size w/o insert (bp) 2636
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Vector typeRNAi ; In situ hybridization
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameslit-1
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SpeciesDugesia dorotocephala
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Insert Size (bp)3812
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ddo_slit-1 was a gift from Alejandro Sánchez (Addgene plasmid # 107636 ; http://n2t.net/addgene:107636 ; RRID:Addgene_107636) -
For your References section:
Hands-On Classroom Activities for Exploring Regeneration and Stem Cell Biology with Planarians. Accorsi A, Williams MM, Ross EJ, Robb SMC, Elliott SA, Tu KC, Sanchez Alvarado A. The American Biology Teacher 79(3):208-223. Mar 2017. 10.1525/abt.2017.79.3.208
