pSLfa1180fa
(Plasmid
#107615)
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Purpose(Empty Backbone) Shuttle vector for easy cloning into Minos/3xP3 transgenesis vectors
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107615 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSL1180
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byReceived from Ernst Wimmer (shared with permission from E. Wimmer).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid has an extensive polylinker (multiple cloning sites) flanked by two AscI and two FseI sites. It provides a convenient intermediate vector for cloning constructs into the transgenesis vectors described in Horn and Wimmer (2000), and into the Minos 3xP3 transgenesis vectors.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLfa1180fa was a gift from Michalis Averof & Ernst Wimmer (Addgene plasmid # 107615 ; http://n2t.net/addgene:107615 ; RRID:Addgene_107615) -
For your References section:
A versatile vector set for animal transgenesis. Horn C, Wimmer EA. Dev Genes Evol. 2000 Dec;210(12):630-7. PubMed 11151300
