pCDNA3.1neo-14-3-3-CLuc
(Plasmid #107611)

• Purpose: measure LATS activity by Luciferase Assay
• Depositing Lab: Xiaolong Yang
• Publication: Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi: 10.1038/s41467-018-03278-w.

Backbone

• Vector backbone: pCDNA3.1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pCDNA3.1hygro-BamHI-CLuc1433-NotI

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To get a good signal this biosensor should be used fresh and pcDNA3.1-MST1-myc might be used as a positive control for the system.

Because of feedback pathways, signaling pathways that increase YAP/TAZ activity sometimes also finally increase LATS and LATS biosensor activity. So, to interpret biosensor signals it is good to always keep the effect of feedback signaling in mind.