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Addgene

pCDNA3.1neo-NLucYAP15
(Plasmid #107610)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107610 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLucYAP15

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To get a good signal this biosensor should be used fresh and pcDNA3.1-MST1-myc might be used as a positive control for the system.

Because of feedback pathways, signaling pathways that increase YAP/TAZ activity sometimes also finally increase LATS and LATS biosensor activity. So, to interpret biosensor signals it is good to always keep the effect of feedback signaling in mind.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1neo-NLucYAP15 was a gift from Xiaolong Yang (Addgene plasmid # 107610 ; http://n2t.net/addgene:107610 ; RRID:Addgene_107610)

  • For your References section:

    A LATS biosensor screen identifies VEGFR as a regulator of the Hippo pathway in angiogenesis. Azad T, Janse van Rensburg HJ, Lightbody ED, Neveu B, Champagne A, Ghaffari A, Kay VR, Hao Y, Shen H, Yeung B, Croy BA, Guan KL, Pouliot F, Zhang J, Nicol CJB, Yang X. Nat Commun. 2018 Mar 13;9(1):1061. doi: 10.1038/s41467-018-03278-w. 10.1038/s41467-018-03278-w PubMed 29535383
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