pU6_T7_INT[6xminimal_Puf1]
(Plasmid
#107608)
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PurposeTransient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107608 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNEB193
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name6x minimal Puf1 motif
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SpeciesSynthetic
- Promoter U6
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pU6_T7_INT[6xminimal_Puf1] was a gift from John Rinn (Addgene plasmid # 107608 ; http://n2t.net/addgene:107608 ; RRID:Addgene_107608) -
For your References section:
Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING). Maass PG, Barutcu AR, Shechner DM, Weiner CL, Mele M, Rinn JL. Nat Struct Mol Biol. 2018 Jan 8. pii: 10.1038/s41594-017-0015-3. doi: 10.1038/s41594-017-0015-3. 10.1038/s41594-017-0015-3 [pii] PubMed 29343869
