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Addgene

pEXP(V5_H2B_APEX2)
(Plasmid #107597)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107597 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST
  • Backbone manufacturer
    invitrogen
  • Modifications to backbone
    quite a few
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-APEX2
  • Promoter CMV Dox inducible
  • Tags / Fusion Proteins
    • V5 (N terminal on insert)
    • H2B (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pCDNA3-flag-Apex2-NES (Addgene plasmid #49386) by Alice Ting. pFC15A_H2B (Promega) a gift from Gordon Hager's lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEXP(V5_H2B_APEX2) was a gift from Markus Hafner (Addgene plasmid # 107597 ; http://n2t.net/addgene:107597 ; RRID:Addgene_107597)

  • For your References section:

    Proximity-CLIP provides a snapshot of protein-occupied RNA elements in subcellular compartments. Benhalevy D, Anastasakis DG, Hafner M. Nat Methods. 2018 Dec;15(12):1074-1082. doi: 10.1038/s41592-018-0220-y. Epub 2018 Nov 26. 10.1038/s41592-018-0220-y PubMed 30478324
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