pCS2-sod1
(Plasmid
#107594)
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PurposeContains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107594 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
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Backbone manufacturer4081
- Total vector size (bp) 4546
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesod1
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)465
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Entrez Genesod1 (a.k.a. Cu/Zn-, Cu/Zn-SOD, ZS, ZSOD, cuz, cuzn, sod(Cu/)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
infusion cloning was used
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-sod1 was a gift from Qing Deng (Addgene plasmid # 107594 ; http://n2t.net/addgene:107594 ; RRID:Addgene_107594) -
For your References section:
Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish. Zhou W, Cao L, Jeffries J, Zhu X, Staiger CJ, Deng Q. Dis Model Mech. 2018 Mar 28;11(3). pii: dmm.033027. doi: 10.1242/dmm.033027. 10.1242/dmm.033027 PubMed 29590639