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Tol2-lyzC-mito-Dendra2
(Plasmid #107590)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107590 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Tol2-lyzC
  • Backbone manufacturer
    Qing Deng
  • Backbone size w/o insert (bp) 5932
  • Total vector size (bp) 6741

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mito-Dendra2
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    801
  • Tag / Fusion Protein
    • mitochondira localization sequence (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

infusion cloning was used

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-lyzC-mito-Dendra2 was a gift from Qing Deng (Addgene plasmid # 107590 ; http://n2t.net/addgene:107590 ; RRID:Addgene_107590)

  • For your References section:

    Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish. Zhou W, Cao L, Jeffries J, Zhu X, Staiger CJ, Deng Q. Dis Model Mech. 2018 Mar 28;11(3). pii: dmm.033027. doi: 10.1242/dmm.033027. 10.1242/dmm.033027 PubMed 29590639
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