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Addgene

pJKW0458
(Plasmid #107587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEarleyGate100
  • Backbone manufacturer
    Keith Earley and Craig Pikaard
  • Backbone size (bp) 11648
  • Modifications to backbone
    p35S:CAS9 in pEarleyGate 100 3xFLAG::NLS::humanized CAS9::NLS (from pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene 42230)) was PCR amplified using pX330 as template, and cloned into Xho1 and Xba1 digested pEARLEYGATE100 by Gibson assembly. Hind III and SbfI sites can be used for cloning in the AtU6-26 sgRNA fragment.
  • Vector type
    Plant Expression, CRISPR
  • Promoter p35S

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJKW0458 was a gift from Jing-Ke Weng (Addgene plasmid # 107587 ; http://n2t.net/addgene:107587 ; RRID:Addgene_107587)
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