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Addgene

pJKW0457
(Plasmid #107586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107586 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Backbone manufacturer
    Messing Lab
  • Backbone size (bp) 2600
  • Modifications to backbone
    AtU6-26 sgRNA fragment was cloned into Hind III SbfI digested pUC18 by Gibson assembly. AtU6-26 sgRNA fragment was synthesized.
  • Vector type
    Plant Expression, CRISPR
  • Promoter AtU6-26

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJKW0457 was a gift from Jing-Ke Weng (Addgene plasmid # 107586 ; http://n2t.net/addgene:107586 ; RRID:Addgene_107586)
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