pXT
(Plasmid
#107574)
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PurposeContains the VGF promoter (about 700 bp) plus 800 bp of 5' untranslated sequence. The HindIII (5')-HincII(3') fragment acts as a NGF-inducible promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107574 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
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Vector typeCloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVGF promoter
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SpeciesR. norvegicus (rat)
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Entrez GeneVgf
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HincII (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXT was a gift from Andrea Levi (Addgene plasmid # 107574 ; http://n2t.net/addgene:107574 ; RRID:Addgene_107574) -
For your References section:
Regulatory elements in the promoter region of vgf, a nerve growth factor-inducible gene. Possenti R, Di Rocco G, Nasi S, Levi A. Proc Natl Acad Sci U S A. 1992 May 1;89(9):3815-9. PubMed 1570299