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Addgene

VGF8a
(Plasmid #107572)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107572 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VGF8a
  • Alt name
    NM_030997
  • Species
    R. norvegicus (rat)
  • Mutation
    partial cDNA (amino acid 65 - 617)
  • Entrez Gene
    Vgf

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: The VGF insert contains a V480M mutation compared to the closest reference sequence. The depositor is unable to confirm what, if any, functional impact this has on the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VGF8a was a gift from Andrea Levi & Bruce Paterson (Addgene plasmid # 107572 ; http://n2t.net/addgene:107572 ; RRID:Addgene_107572)

  • For your References section:

    Molecular cloning of a gene sequence regulated by nerve growth factor. Levi A, Eldridge JD, Paterson BM. Science. 1985 Jul 26;229(4711):393-5. PubMed 3839317
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