996 pSG5L HA PTEN S380A
(Plasmid #10755)

• Depositing Lab: William Sellers
• Publication: Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Backbone

• Vector backbone: pSG5L-HA
• Backbone size w/o insert (bp): 4100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTEN
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Mutation: S380A
• Entrez Gene: PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry