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Addgene

pAAV-syn-AICD-IRES-hrGFP
(Plasmid #107548)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107548 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-syn-GFP
  • Backbone manufacturer
    Addgene plasmid #58867
  • Modifications to backbone
    GFP was replaced by AICD-IRES-hrGFP between the BamHI and EcoRI site.
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AICD
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Entrez Gene
    APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
  • Promoter human synapsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CTGCGTATGAGTGCAAG
  • 3′ sequencing primer cagcgtatccacatagcg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-syn-AICD-IRES-hrGFP was a gift from Hélène Marie (Addgene plasmid # 107548 ; http://n2t.net/addgene:107548 ; RRID:Addgene_107548)

  • For your References section:

    Physiological and pathophysiological control of synaptic GluN2B-NMDA receptors by the C-terminal domain of amyloid precursor protein. Pousinha PA, Mouska X, Raymond EF, Gwizdek C, Dhib G, Poupon G, Zaragosi LE, Giudici C, Bethus I, Pacary E, Willem M, Marie H. Elife. 2017 Jul 6;6. doi: 10.7554/eLife.25659. 10.7554/eLife.25659 PubMed 28682239
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