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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10753 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSG5L-HA
- Backbone size w/o insert (bp) 4100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePTEN
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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MutationA4: S380A, T382A, T383A, S385A
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Entrez GenePTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
977 pSG5L HA PTEN A4 was a gift from William Sellers (Addgene plasmid # 10753 ; http://n2t.net/addgene:10753 ; RRID:Addgene_10753) -
For your References section:
Phosphorylation of the PTEN tail regulates protein stability and function. Vazquez F, Ramaswamy S, Nakamura N, Sellers WR. Mol Cell Biol. 2000 Jul . 20(14):5010-8. 10.1128/MCB.20.14.5010-5018.2000 PubMed 10866658
Map uploaded by the depositor.
