pCEP4-SERTG484E-myc
(Plasmid
#107471)
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PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G484E decreased transport and surface expression relative to wildtype
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107471 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCEP4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10410
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSerotonin Transporter (SERT)
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Alt nameSLC6A4
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SpeciesH. sapiens (human)
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Insert Size (bp)1985
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MutationG484E
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Entrez GeneSLC6A4 (a.k.a. 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, SERT1, hSERT)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NHE1 (not destroyed)
- 3′ cloning site XHO1 (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer EBV Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCEP4-SERTG484E-myc was a gift from Erik Procko (Addgene plasmid # 107471 ; http://n2t.net/addgene:107471 ; RRID:Addgene_107471) -
For your References section:
Deep Mutagenesis of a Transporter for Uptake of a Non-Native Substrate Identifies Conformationally Dynamic Regions. Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E. bioRxiv 2021.04.19.440442 10.1101/2021.04.19.440442
