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Addgene

pCEP4-NET-myc
(Plasmid #107458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10410
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Norepinephrine Transporter (NET)
  • Alt name
    SLC6A2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1953
  • Entrez Gene
    SLC6A2 (a.k.a. NAT1, NET, NET1, SLC6A5)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NHE1 (not destroyed)
  • 3′ cloning site XHO1 (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer EBV Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-NET-myc was a gift from Erik Procko (Addgene plasmid # 107458 ; http://n2t.net/addgene:107458 ; RRID:Addgene_107458)

  • For your References section:

    Deep Mutagenesis of a Transporter for Uptake of a Non-Native Substrate Identifies Conformationally Dynamic Regions. Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E. bioRxiv 2021.04.19.440442 10.1101/2021.04.19.440442
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