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Addgene

HBT-CPK32ac-Flag
(Plasmid #107431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    NEB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CPK32
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    978
  • Entrez Gene
    CPK32 (a.k.a. ATCPK32, CALCIUM-DEPENDENT PROTEIN KINASE 32, CDPK32, CPK32)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results confirmed that this plasmid contains amino acids 1-326 of the CPK32 translation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HBT-CPK32ac-Flag was a gift from Jen Sheen (Addgene plasmid # 107431 ; http://n2t.net/addgene:107431 ; RRID:Addgene_107431)

  • For your References section:

    Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks. Liu KH, Niu Y, Konishi M, Wu Y, Du H, Sun Chung H, Li L, Boudsocq M, McCormack M, Maekawa S, Ishida T, Zhang C, Shokat K, Yanagisawa S, Sheen J. Nature. 2017 May 18;545(7654):311-316. doi: 10.1038/nature22077. Epub 2017 May 10. 10.1038/nature22077 PubMed 28489820
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