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Addgene

pMB1-spect-PthrC3_25-eGFP
(Plasmid #107413)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107413 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMB1-spect-PthrC3_25
  • Backbone size w/o insert (bp) 711
  • Total vector size (bp) 3291
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use strain BL21(DE3) for expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • GenBank ID
    KY014123.1
  • Promoter PthrC3_25

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer agcttttcattctgactgca
  • 3′ sequencing primer GGTTGTTACCTCGTTACCTT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB1-spect-PthrC3_25-eGFP was a gift from Kang Zhou (Addgene plasmid # 107413 ; http://n2t.net/addgene:107413 ; RRID:Addgene_107413)

  • For your References section:

    Short, auto-inducible promoters for well-controlled protein expression in Escherichia coli. Anilionyte O, Liang H, Ma X, Yang L, Zhou K. Appl Microbiol Biotechnol. 2018 Aug;102(16):7007-7015. doi: 10.1007/s00253-018-9141-z. Epub 2018 Jun 8. 10.1007/s00253-018-9141-z PubMed 29948110
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