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Addgene

WT PBRM1- TRIPZ-neo
(Plasmid #107406)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107406 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTRIPZ-neo
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Please grow transformed cells for 2+ days
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PBRM1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PBRM1 (a.k.a. BAF180, PB1, RCC, SMARCH1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is unstable and likely to recombine when grown. Upon receipt it is essential to screen multiple colonies to confirm the intact plasmid is present. Recombined products are not expected to impact viral packaging if the full length plasmid is present.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WT PBRM1- TRIPZ-neo was a gift from William Kaelin (Addgene plasmid # 107406)

  • For your References section:

    Inactivation of the PBRM1 tumor suppressor gene amplifies the HIF-response in VHL-/- clear cell renal carcinoma. Gao W, Li W, Xiao T, Liu XS, Kaelin WG Jr. Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1027-1032. doi: 10.1073/pnas.1619726114. Epub 2017 Jan 12. 10.1073/pnas.1619726114 PubMed 28082722
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