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Addgene

shPBRM1#32-Tet-pLKO-puro
(Plasmid #107399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Tet-pLKO-puro
  • Backbone manufacturer
    Dmitri Wiederschain (Addgene plasmid # 21915)
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    shRNA targeting PBRM1
  • gRNA/shRNA sequence
    ccggGCACTCAGCTATACCACAAaactcgagttTTGTGGTATAGCTGAGTGCttttt
  • Species
    H. sapiens (human)
  • Entrez Gene
    PBRM1 (a.k.a. BAF180, PB1, RCC, SMARCH1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    shPBRM1#32-Tet-pLKO-puro was a gift from William Kaelin (Addgene plasmid # 107399 ; http://n2t.net/addgene:107399 ; RRID:Addgene_107399)

  • For your References section:

    Inactivation of the PBRM1 tumor suppressor gene amplifies the HIF-response in VHL-/- clear cell renal carcinoma. Gao W, Li W, Xiao T, Liu XS, Kaelin WG Jr. Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1027-1032. doi: 10.1073/pnas.1619726114. Epub 2017 Jan 12. 10.1073/pnas.1619726114 PubMed 28082722
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