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Addgene

plenti-px330-CRBN-T1-pGK-Pur
(Plasmid #107382)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107382 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    plenti-px330-pGK-Pur
  • Vector type
    Mammalian Expression, Lentiviral ; CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sgRNA targeting CRBN
  • gRNA/shRNA sequence
    TCCTGCTGATCTCCTTCGC
  • Species
    H. sapiens (human)
  • Entrez Gene
    CRBN (a.k.a. MRT2, MRT2A)
  • Promoter U6

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plenti-px330-CRBN-T1-pGK-Pur was a gift from William Kaelin (Addgene plasmid # 107382 ; http://n2t.net/addgene:107382 ; RRID:Addgene_107382)

  • For your References section:

    The myeloma drug lenalidomide promotes the cereblon-dependent destruction of Ikaros proteins. Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK, Bradner JE, Kaelin WG Jr. Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. Epub 2013 Nov 29. 10.1126/science.1244917 PubMed 24292623
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