815 pSG5L HA PTEN delta274-342
(Plasmid #10738)

• Depositing Lab: William Sellers
• Publication: Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5.

Backbone

• Vector backbone: pSG5L-HA
• Backbone size w/o insert (bp): 4100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTEN
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: Deletion of amino acids 274-342.
• Entrez Gene: PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry