VGluT1-SNAPtag
(Plasmid
#107371)
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PurposeSynaptic vesicle protein VGluT1 fused to the SNAPtag domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107371 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCaggs
- Backbone size w/o insert (bp) 2361
- Total vector size (bp) 7347
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVesicular Glutamate transporter 1
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Alt nameBNPI
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Alt nameSlc17a7
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SpeciesR. norvegicus (rat)
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Entrez GeneSlc17a7 (a.k.a. BNPI, Vglut1)
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Tag
/ Fusion Protein
- SNAPtag, 104 aa before C term
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VGluT1-SNAPtag was a gift from David Perrais (Addgene plasmid # 107371 ; http://n2t.net/addgene:107371 ; RRID:Addgene_107371) -
For your References section:
Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis. Martineau M, Somasundaram A, Grimm JB, Gruber TD, Choquet D, Taraska JW, Lavis LD, Perrais D. Nat Commun. 2017 Nov 10;8(1):1412. doi: 10.1038/s41467-017-01752-5. 10.1038/s41467-017-01752-5 [pii] PubMed 29123102