VAMP2-pHuji
(Plasmid #107369)

• Purpose: Synaptic vesicle protein VAMP2 fused to the red FP pH sensor pHuji
• Depositing Lab: David Perrais
• Publication: Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi: 10.1038/s41467-017-01752-5.

Backbone

• Vector backbone: pCMV
• Backbone size w/o insert (bp): 3740
• Total vector size (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VAMP2
• Alt name: Synaptobrevin 2
• Species: R. norvegicus (rat)
• GenBank ID: NM_012663.2
• Entrez Gene: Vamp2 (a.k.a. RATVAMPB, RATVAMPIR, SYB, Syb2)
• Tag / Fusion Protein:
  • pHuji (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: na
• 3′ sequencing primer: na

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Lab of Justin Taraska, NIH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry