pNP1 sfGFP toehold switch
(Plasmid #107355)

• Purpose: Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed
• Depositing Lab: James Collins
• Publication: Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. eCollection 2018 Aug.

Backbone

• Vector backbone: pNP1
• Backbone manufacturer: New England Biolabs (cloning vector)
• Backbone size w/o insert (bp): 2202
• Total vector size (bp): 3006
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: toehold 7 + sfGFP
• Insert Size (bp): 804
• Promoter: T7

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCCGCGAAATTAATACGACTCACTATAGG
• 3′ sequencing primer: AGTGCCAAGCTTCCGGATATAGTTC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: toehold - Green, A.A., Silver, P.A., Collins, J.J., and Yin, P. (2014). Toehold switches: de-novo-designed regulators of gene expression. Cell 159, 925–939. sfGFP - Michael Jewett Lab (Addgene 102634)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry