pNP1 eforRed toehold switch
(Plasmid #107353)

• Purpose: Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed
• Depositing Lab: James Collins
• Publication: Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. eCollection 2018 Aug.

Backbone

• Vector backbone: pNP1
• Backbone manufacturer: New England Biolabs (cloning vector)
• Backbone size w/o insert (bp): 2202
• Total vector size (bp): 2967
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: toehold switch 1 + eforRed
• Insert Size (bp): 765
• Promoter: T7

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCCGCGAAATTAATACGACTCACTATAGG
• 3′ sequencing primer: AGTGCCAAGCTTCCGGATATAGTTC

Resource Information

• Supplemental Documents:
  • pNP1 eforRed toehold switch.gb
• A portion of this plasmid was derived from a plasmid made by: eforRed - iGEM Part BBa_K592012 toehold - Green, A.A., Silver, P.A., Collins, J.J., and Yin, P. (2014). Toehold switches: de-novo-designed regulators of gene expression. Cell 159, 925–939.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry