pLVX-UBE3B-BirA-Puro
(Plasmid #107352)

• Purpose: Expresses UBE3B fused to BirA in mammalian cells.
• Depositing Lab: Robert Sobol
• Publication: Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 10.1074/jbc.M116.766824.

Backbone

• Vector backbone: pLVX-GWB-IRES-Puro
• Backbone size w/o insert (bp): 8247
• Total vector size (bp): 12401
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: UBE3B-BirA
• Species: H. sapiens (human)
• Insert Size (bp): 4100
• Entrez Gene: UBE3B (a.k.a. BPIDS, KOS)
• Promoter: CMV
• Tag / Fusion Protein:
  • Biotin ligase (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13
• 3′ sequencing primer: M13

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA