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Addgene

pLVX-BirA-UBE3B-Puro
(Plasmid #107351)

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Item Catalog # Description Quantity Price (USD)
Plasmid 107351 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pLVX-GWB-IRES-Puro
  • Backbone size w/o insert (bp) 8247
  • Total vector size (bp) 12401
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BirA-UBE3B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4100
  • Entrez Gene
    UBE3B (a.k.a. BPIDS, KOS)
  • Promoter CMV
  • Tag / Fusion Protein
    • Biotin ligase (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-BirA-UBE3B-Puro was a gift from Robert Sobol (Addgene plasmid # 107351 ; http://n2t.net/addgene:107351 ; RRID:Addgene_107351)

  • For your References section:

    UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 Ubiquitin Ligase. Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, Brodsky JL, Sobol RW. J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 10.1074/jbc.M116.766824. 10.1074/jbc.M116.766824 PubMed 28003368
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