pCT-CMV-UBE3BΔHECT-copGFP-Puro
(Plasmid #107343)

• Purpose: Expresses UBE3B deletion of HECT domain fused to copGFP in mammalian cells.
• Depositing Lab: Robert Sobol
• Publication: Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 10.1074/jbc.M116.766824.

Backbone

• Vector backbone: pCYTO-CMV-MCS-copGFP-EF1-Puro
• Backbone manufacturer: System Biosciences
• Backbone size w/o insert (bp): 7400
• Total vector size (bp): 10393
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: UBE3B(ΔHECT)-copGFP
• Species: H. sapiens (human)
• Insert Size (bp): 3046
• Mutation: deletion of HECT domain (757-end)
• Entrez Gene: UBE3B (a.k.a. BPIDS, KOS)
• Promoter: CMV
• Tag / Fusion Protein:
  • copGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: ATTCTAGACACCATGTTCACCCTGTCTCAGACCTCG
• 3′ sequencing primer: ATCGGATCCAATCCCCACTGGTTGTCTTGAACAGATT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Industry MTA
  • Evrogen Ancillary Agreement for FP Distribution to Industry