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pEXP5-aHL_spytag_his
(Plasmid #107334)

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Item Catalog # Description Quantity Price (USD)
Plasmid 107334 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEXP5-NT
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2745
  • Total vector size (bp) 3636
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    alpha-hemolysin-SpyTag-6xHis
  • Alt name
    AHL-SpyTag-His
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEXP5-aHL_spytag_his was a gift from George Church (Addgene plasmid # 107334 ; http://n2t.net/addgene:107334 ; RRID:Addgene_107334)

  • For your References section:

    Design and characterization of a nanopore-coupled polymerase for single-molecule DNA sequencing by synthesis on an electrode array. Stranges PB, Palla M, Kalachikov S, Nivala J, Dorwart M, Trans A, Kumar S, Porel M, Chien M, Tao C, Morozova I, Li Z, Shi S, Aberra A, Arnold C, Yang A, Aguirre A, Harada ET, Korenblum D, Pollard J, Bhat A, Gremyachinskiy D, Bibillo A, Chen R, Davis R, Russo JJ, Fuller CW, Roever S, Ju J, Church GM. Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):E6749-E6756. doi: 10.1073/pnas.1608271113. Epub 2016 Oct 11. 10.1073/pnas.1608271113 PubMed 27729524
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