pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS
(Plasmid
#107324)
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PurposeExpresses SpCas9 fused to NmCas9 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107324 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCSDest2
- Total vector size (bp) 11837
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpCas9-NmCas9 fusion
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SpeciesSynthetic; S. pyogenes, N. meningitidis
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Insert Size (bp)7743
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MutationSpCas9 is fused to NmCas9
- Promoter CMV IE94
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Tags
/ Fusion Proteins
- NLS (N terminal on insert)
- NLS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Sp6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS was a gift from Scot Wolfe (Addgene plasmid # 107324 ; http://n2t.net/addgene:107324 ; RRID:Addgene_107324) -
For your References section:
Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing. Bolukbasi MF, Liu P, Luk K, Kwok SF, Gupta A, Amrani N, Sontheimer EJ, Zhu LJ, Wolfe SA. Nat Commun. 2018 Nov 19;9(1):4856. doi: 10.1038/s41467-018-07310-x. 10.1038/s41467-018-07310-x PubMed 30451839
