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Addgene

pCSD_SpCas9MT3-FKBP_SL
(Plasmid #107302)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107302 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSDest2
  • Backbone size w/o insert (bp) 4128
  • Total vector size (bp) 8637
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    4512
  • Mutation
    R1335K and fused to FKBP
  • Promoter CMV IE94
  • Tag / Fusion Protein
    • FKBP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSD_SpCas9MT3-FKBP_SL was a gift from Scot Wolfe (Addgene plasmid # 107302 ; http://n2t.net/addgene:107302 ; RRID:Addgene_107302)
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