pCSD_N-SpCas9-1-573-FRB-1xNLS
(Plasmid
#107298)
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PurposeExpresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107298 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCSDest2
- Backbone size w/o insert (bp) 4117
- Total vector size (bp) 6180
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTruncated SpCas9 (aa 1-573) fused to FRB
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SpeciesSynthetic; S. pyogenes
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Insert Size (bp)2055
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MutationTruncated SpCas9 (aa 1-573)
- Promoter CMV IE94
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Tags
/ Fusion Proteins
- SV40 NLS (C terminal on insert)
- FRB (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer Sp6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSD_N-SpCas9-1-573-FRB-1xNLS was a gift from Scot Wolfe (Addgene plasmid # 107298 ; http://n2t.net/addgene:107298 ; RRID:Addgene_107298)
