RINS1(mut)
(Plasmid #107291)

• Purpose: Plasmid for the expression of the inactive mutant of RINS1 sensor
• Depositing Lab: Dmytro Yushchenko
• Publication: Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-531.e4. doi: 10.1016/j.chembiol.2017.03.001. Epub 2017 Mar 30.

Backbone

• Vector backbone: C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin, mCherry, sfGFP

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RINS1(mut)
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1812
• Mutation: two Arg/Ser mutations at the beginning and in the end of the C-chain of insulin to prevent the cleavege of C-peptide upon insulin maturation
• Promoter: CMV
• Tag / Fusion Protein:
  • sfGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F: CGCAAATGGGCGGTAGGCGTG; pEGFP-FP:TTTAGTGAACCGTCAGATC
• 3′ sequencing primer: pEGFP_C2-RP: TTTAAAGCAAGTAAAACCTC; SV40pA-R: GAAATTTGTGATGCTATTGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry