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Addgene

RINS1
(Plasmid #107290)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107290 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin, mCherry, sfGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RINS1
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1812
  • Promoter CMV
  • Tag / Fusion Protein
    • sfGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F: CGCAAATGGGCGGTAGGCGTG; pEGFP-FP:TTTAGTGAACCGTCAGATC
  • 3′ sequencing primer pEGFP_C2-RP: TTTAAAGCAAGTAAAACCTC; SV40pA-R: GAAATTTGTGATGCTATTGC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RINS1 was a gift from Dmytro Yushchenko (Addgene plasmid # 107290 ; http://n2t.net/addgene:107290 ; RRID:Addgene_107290)

  • For your References section:

    A Ratiometric Sensor for Imaging Insulin Secretion in Single beta Cells. Schifferer M, Yushchenko DA, Stein F, Bolbat A, Schultz C. Cell Chem Biol. 2017 Apr 20;24(4):525-531.e4. doi: 10.1016/j.chembiol.2017.03.001. Epub 2017 Mar 30. 10.1016/j.chembiol.2017.03.001 PubMed 28366620
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