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Addgene

TrubIFN-γ
(Plasmid #107288)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107288 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET26b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5360
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Interferon gamma
  • Species
    Takifugu rubripes (fish)
  • Insert Size (bp)
    507
  • Promoter T7 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site CATATG (not destroyed)
  • 3′ cloning site CTCGAG (not destroyed)
  • 5′ sequencing primer pETup2
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TrubIFN-γ was a gift from Bohdan Schneider (Addgene plasmid # 107288 ; http://n2t.net/addgene:107288 ; RRID:Addgene_107288)

  • For your References section:

    Interferons type II and their receptors R1 and R2 in fish species: Evolution, structure, and function. Zahradnik J, Kolarova L, Parizkova H, Kolenko P, Schneider B. Fish Shellfish Immunol. 2018 May 6;79:140-152. doi: 10.1016/j.fsi.2018.05.008. 10.1016/j.fsi.2018.05.008 PubMed 29742458
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