OnilIFN-γ
(Plasmid
#107287)
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PurposePlasmid can be used for secretion of fish Interferon gamma
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107287 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET26b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5360
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameInterferon gamma
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SpeciesOreochromis niloticus (fish)
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Insert Size (bp)555
- Promoter T7 promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site CATATG (not destroyed)
- 3′ cloning site CTCGAG (not destroyed)
- 5′ sequencing primer pETup2
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OnilIFN-γ was a gift from Bohdan Schneider (Addgene plasmid # 107287 ; http://n2t.net/addgene:107287 ; RRID:Addgene_107287) -
For your References section:
Interferons type II and their receptors R1 and R2 in fish species: Evolution, structure, and function. Zahradnik J, Kolarova L, Parizkova H, Kolenko P, Schneider B. Fish Shellfish Immunol. 2018 May 6;79:140-152. doi: 10.1016/j.fsi.2018.05.008. 10.1016/j.fsi.2018.05.008 PubMed 29742458