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Addgene

498 pSG5L HA RB del663 (NAAIRS)
(Plasmid #10728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5L
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RB del663
  • Alt name
    retinoblastoma
  • Alt name
    RB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2800
  • Mutation
    residues 664 to 669 were replaced by NAAIRS, a flexible polylinker
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

del663 created by site-directed mutagenesis using this primer: GCCTATCTCCGGCTAaatgctgctatacgatcgCTTCTGTCTGAGCAC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    498 pSG5L HA RB del663 (NAAIRS) was a gift from William Sellers (Addgene plasmid # 10728 ; http://n2t.net/addgene:10728 ; RRID:Addgene_10728)

  • For your References section:

    Stable binding to E2F is not required for the retinoblastoma protein to activate transcription, promote differentiation, and suppress tumor cell growth. Sellers WR, Novitch BG, Miyake S, Heith A, Otterson GA, Kaye FJ, Lassar AB, Kaelin WG. Genes Dev. 1998 Jan 1. 12(1):95-106. 10.1101/gad.12.1.95 PubMed 9420334
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