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p3-HPRT1-S104R-PdTK-mCh
(Plasmid #107276)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107276 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Selectable markers
    Puromycin ; thymidine kinase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    HPRT genomic DNA
  • Species
    H. sapiens (human)
  • Mutation
    c.306G>T, c.312C>A
  • Entrez Gene
    HPRT1 (a.k.a. HGPRT, HPRT)

Gene/Insert 2

  • Gene/Insert name
    puro-deltaTK; CAG::mCherry
  • Alt name
    MhAX cassette
  • Insert Size (bp)
    5119

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3-HPRT1-S104R-PdTK-mCh was a gift from Knut Woltjen (Addgene plasmid # 107276 ; http://n2t.net/addgene:107276 ; RRID:Addgene_107276)

  • For your References section:

    Microhomology-assisted scarless genome editing in human iPSCs. Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K. Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y. 10.1038/s41467-018-03044-y PubMed 29507284
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