pSmartBAC-S
(Plasmid #107268)

• Purpose: (Empty Backbone) Broad host range bacterial artificial chromosome vector
• Depositing Labs: Ron Godiska, Mark Liles
• Publication: Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332. doi: 10.1021/acs.jnatprod.7b00903. Epub 2018 Jun 13.

Backbone

• Vector backbone: pSmartBAC (EU101022.1)
• Backbone manufacturer: Lucigen Corporation
• Backbone size (bp): 7648
• Modifications to backbone: We first restriction digested pSmartBAC (EU101022.1) with ApaI, end-repaired the DNA to make it blunt and dephosphorylated the digested pSmartBAC vector ends. A synthesized and blunt-ended gene cassette that contained the ɸC31 integrase (int), attachment site (attP) and aac3(IV) that encodes for apramycin-resistance (4,793bp) was ligated to the linearized pSmartBAC-S and apramycin-resistant transformants in E. coli 10G were selected.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Arabinose induction of copy number
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pSmartBAC-S.gbk
• A portion of this plasmid was derived from a plasmid made by: The gene cassette was synthesized by Dr. ChengCang Wu, formerly of Lucigen. More information if needed could be obtained directly from him at

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also carries apramycin resistance cassette. Selection for chloramphenicol and/or apramycin resistance will maintain the plasmid.