mCherry-eDHFR-Cdc42Q61L
(Plasmid
#107267)
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PurposemCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107267 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIVT
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Backbone manufacturerCarmen Williams (Addgene plasmid #32374)
- Total vector size (bp) 4850
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer mCherry-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGuillaume Halet at Institut de Génétique et Développement de Rennes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-eDHFR-Cdc42Q61L was a gift from Michael Lampson (Addgene plasmid # 107267 ; http://n2t.net/addgene:107267 ; RRID:Addgene_107267) -
For your References section:
Spindle asymmetry drives non-Mendelian chromosome segregation. Akera T, Chmatal L, Trimm E, Yang K, Aonbangkhen C, Chenoweth DM, Janke C, Schultz RM, Lampson MA. Science. 2017 Nov 3;358(6363):668-672. doi: 10.1126/science.aan0092. 10.1126/science.aan0092 PubMed 29097549
